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KMID : 0545120120220101359
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Journal of Microbiology and Biotechnology
2012 Volume.22 No. 10 p.1359 ~ p.1366
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Biocontrol of Anthracnose in Pepper Using Chitinase, β-1,3 Glucanase, and 2-Furancarboxaldehyde Produced by Streptomyces cavourensis SY224
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Lee So-Youn
Hamisi Tindwa
Lee Yong-Seong
Kyaw Wai Naing
Hong Seong-Hyun
Nam Yi
Kim Kil-Yong
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Abstract
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A strain of Streptomyces cavourensis subsp. cavourensis (coded as SY224) antagonistic to Colletotrichum gloeosporioides infecting pepper plants was isolated. SY224 produced lytic enzymes such as chitinase, β-1,3-glucanase, lipase, and protease in respective assays. To examine for antifungal activity, the treatments amended with the nonsterilized supernatant resulted in the highest growth inhibition rate of about 92.9% and 87.4% at concentrations of 30% and 10%, respectively. However, the sterilized treatments (autoclaved or chloroform treated) gave a lowered but significant inhibitory effect of about 63.4% and 62.6% for the 10% supernatant concentration, and 75.2% and 74.8% for the of 30% supernatant concentration in the PDA agar medium, respectively, indicative of the role of a nonprotein, heat stable compound on the overall effect. This antifungal compound, which inhibited spore germination and altered hyphal morphology, was extracted by EtOAc and purified by ODS, silica gel, Sephadex LH-20 column, and HPLC, where an active fraction was confirmed to be 2-furancarboxaldehyde by GS-CI MS techniques. These results suggested that SY224 had a high potential in the biocontrol of anthracnose in pepper, mainly due to a combined effect of lytic enzymes and a non-protein, heatstable antifungal compound, 2-furancarboxaldehyde.
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KEYWORD
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Antifungal activity, β-1, 3 glucanase, chitinase, 2-furancarboxaldehyde, Streptomyces cavourensis
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